Download Biotechnology, 2nd Edition, Volume 2: Genetic Fundamentals by Alfred Puhler PDF

By Alfred Puhler

With no adequate wisdom of classical and molecular genetics in addition to of genetic engineering, it isn't attainable to totally unterstand biotechnological methods. transparent, concise and finished, quantity 2 offers scientists all of the details they should perform learn and creation during this box. partly I of the booklet the classical genetic innovations suitable to biotechnology are provided. half II highlights the new advances in molecular genetics that have revolutionized our wisdom within the box. half III bargains with the genetic engineering of microorganisms, and components IV and V pay attention to crops and animals - multicellular organsims which require specific genetic engineering innovations. within the ultimate half VI biosafety strategies in smooth biotechnology are taken care of. subject matters integrated are: Mutagenesis/ Genetic trade approaches/ mobile Fusion/ Gene Mapping/ Transposable parts/ Gene Expression/ Synthesis of Oligonucleotides/ Polymerase Chain response (PCR)/ Transposons/ Transformation Vectors/ Recombinant Proteins/ Gene Amplification/ Transgenic vegetation and Animals/ Biosafety innovations

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Res. 136, 185199. DEMEREC,M. (1937), Frequency of spontaneous mutations in certain stocks of Drosophrla melanogaster, Genetics 22, 469-478. DEN ENGELSE,L. , DE BRIJ, R. , MENKVELD, G. J . (1987), 0’- and 04-Ethylthymine and the ethyl phosphotriester dTp(Et)dT are highly persistent DNA modifications in slowly dividing tissues of the ethylnitrosourea treated rats, Carcinogenesis 2, 751-757. DHILLON,E. K. , DHILLON,T. S. (1974), N-methyl-N’-nitro-N-nitrosoguanidine and hydroxylamine induced mutants in the rII region of the phage T4, Mutat.

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KNILL-JONES,J. -C. Escherichia coli with an unusually high rate of (1982), Kinetic basis of spontaneous misinsertion auxotrophic mutation, J. Bacteriol. 70, 588-595. frequencies, proofreading specificities and cost of proofreading by DNA polymerases of GOODMAN,M. , GORE, W. C. Escherichia coli, J. Mol. Biol. 156, 37-5 1. (1977), 2-Aminopurine-induced mutagenesis in FRAM, R. , MARINUS,M. G. T4 bacteriophage: a model relating mutation fre(1986), Mutagenesis and repair of DNA damage quency to 2-aminopurine incorporation in DNA, caused by nitrogen mustard, N,N’-bis(2-chloroProc.

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